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Test Code GTT + # of Hours Glucose Tolerance Test

Performing Laboratory

Chilton Hospital Laboratory-Chemistry

Methodology

Colorimetric

Specimen Requirements

Patient Preparation:
1. Patient should be instructed to eat and drink nothing except water after midnight.

2. Patient should be instructed to refrain from smoking and drinking coffee just before and during test.

 

Performance of Test:

1. Physician orders specific amount of Glucola and length of test.

2. Draw blood for a fasting glucose. Do not perform test if patient is not fasting or fasting glucose is 160 mg/dL.

3. Give patient standard glucose solution as follows:

Glucose Tolerance Oral Dose
Age/Weight Amount of Glucose Solution
Adults or children >12 years 100 g (10.0 ounces)
Children under 12 years:
>95 pounds 7.5 ounces
82-94 pounds 7.0 ounces
70-81 pounds 6.0 ounces
57-69 pounds 5.0 ounces
44-56 pounds 4.0 ounces
31-43 pounds 3.0 ounces
<30 pounds Consult pathologist at 973-831-5046

Calculation:  1 pound = 0.45 kg

1.75 g (0.175 ounce) of glucose solution per kg

4. Draw specimen(s) at specified times.

 

Specimen Collection:

Specimen Type: Plasma or serum
Container/Tube: Light green top (lithium heparin gel), gold or tiger top (serum gel)

Specimen Volume: Full tube

Collection Instructions: Label tube with time of draw.

Specimen Transport Temperature

Ambient

Reference Values

Fasting: 70-105 mg/dL

30 minutes post-dose: 75-140 mg/dL

1 hour post-dose: 130-160 mg/dL

1.5 hours post-dose: 70-115 mg/dL

2 hours post-dose: 70-115 mg/dL

≥3 hours post-dose: 65-105 mg/dL

Day(s) Test Set Up

Monday through Friday: inpatients

Saturday: outpatients only, when scheduled with laboratory

Turnaround Time:

Routine-8 hours